The Kew Gardens 50p, owned by a coin collector in Worcestershire, has sold on eBay for £275.
The Royal Mint has called the coin “the most sought-after” by collectors due to its rarity.
The 50p, issued by the Royal Mint in 2009, is believed by many collectors to be so rare today as it had a low mintage of just 210,000 due to the low demand for 50p coins when it was minted.
Queen Elizabeth II features on the head of the coin, and the reverse is dominated by the iconic Chinese Pagoda at Kew, with a leafy climber wrapped around the tower.
The coin sold on eBay had its value verified by the Numismatic Guaranty Corporation, who graded the coin with a PF69 rating.
In coin collecting, an item with a PF70 rating is considered to be perfect and is the highest grading a coin can receive.
A PF69 rating is the second highest grading afforded by the NGC and denotes an almost perfect coin and contains virtually unnoticeable flaws
However, due to their popularity, even the poorest condition Kew Gardens 50p coins are still sold at a huge price compared to their value.
A spokesperson for Copes Coins said: “A poor condition Kew Gardens 50p could still be worth around £160 to £180.
“In contrast, a circulated in great condition could fetch anything from £200 to £250 and uncirculated and brilliant uncirculated would have a value of much more.”